JOB DESCRIPTION:
• Design and engineer Client cell lines in iPSCs, tumor cell lines, and other immortalized and primary cell types to support allogeneic cell therapy programs
• Generate stable engineered cell lines using lentiviral transduction and other viral vector systems
• Design genetic constructs and mRNA for stable and transient gene expression, including knock-in, knockout, and reporter strategies
• Perform transient mRNA-based expression to rapidly prototype and validate cell engineering strategies
• Establish clonal engineered cell lines through cell sorting (FACS) and single-cell cloning
• Develop, optimize, and execute cell-based assays to characterize engineered cells, including live-cell analysis on the Incucyte platform
• Design and run in-depth, multi-parameter flow cytometry panels to phenotype engineered cells and assess immune checkpoint receptor expression
• Evaluate the functional impact of genetic modifications on engineered cell phenotype and function
• Design and execute experiments, analyze data, and interpret results to evaluate the performance of engineered cells in vitro
• Communicate experimental data effectively through written reports and oral presentations.
• Demonstrate flexibility and motivation to learn and apply new technologies for cell engineering and assay development.
QUALIFICATIONS:
Scientist, Cell Engineering and Assay Development
As a Scientist in Cell Engineering and Assay Development, you will engineer Client cell lines and develop the in vitro assays used to characterize them in support of our allogeneic cell therapy programs. You will design genetic constructs and mRNA, generate engineered cell lines using viral vectors and transient mRNA-based expression, and build cell-based assays to evaluate the function of engineered cells. You will work closely with cross-functional teams to advance cell therapy programs from discovery through preclinical development.
Basic Requirements:
Bachelor's degree in Cell Biology, Molecular Biology, Immunology, or a related field plus 5 years of relevant experience in biotechnology, pharmaceutical industry, or academic research.
Master's degree in Cell Biology, Molecular Biology, Immunology, or a related field plus 2 years of relevant experience in biotechnology, pharmaceutical industry, or academic research.
Hands-on experience with lentiviral cell line generation is required.
Additional Skills/Preferences:
• Experience engineering iPSCs and tumor cell lines
• Proficiency in construct design and mRNA design
• Experience with cell engineering using viral vectors and transient mRNA-based expression
• In-depth flow cytometry expertise, including panel design, cell sorting (FACS), and multi-parameter data analysis
• Working knowledge of immune checkpoint receptor biology
• Experience with live-cell analysis platforms such as Incucyte and xCELLigence
• Proficient in developing in vitro cell-based assays, including work with human cell lines and primary cells
• Strong analytical, problem-solving, and decision-making skills.
• Excellent interpersonal and communication skills.
• Ability to work independently and collaboratively within a team environment.
